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. Author manuscript; available in PMC: 2013 Jan 5.
Published in final edited form as: Virology. 2011 Oct 30;422(1):59–69. doi: 10.1016/j.virol.2011.09.033

Table 1.

Sequencing results of the DNA bands obtained from 5'/3' RACE and RT-PCR

Lane Analysis Primers* Bands Identified transcripts*
1 5' RACE d(G)16-R nt960 a P6 nt262-960
2 5' RACE d(G)16-R nt3840 a P38 nt1950-2060Δ2309-2367Δ3329-3840
b P38 nt1950-2013Δ3329-3840
3 RT-PCR F nt283-R nt2060 a P6 nt283-2060
b P6 nt283-418Δ1630-2060
4 RT-PCR F nt1441-R nt2354 a non-specific
b P6 nt1441-2354
c P6 nt1441-2060Δ2309-2354
5 5' RACE d(T)18V-R nt3440 a P38 nt1950-2060Δ2309-2367Δ3329-3440
b P38 nt1950-2013Δ3329-3440
c Primer dimers
6 RT-PCR F nt283-R nt3440 a P6 nt283-2060Δ2309-2367Δ3329-3440
b P6 nt283-418Δ1630-2060Δ2309-2367Δ3329-3440
c P6 nt283-418Δ1630-2013Δ3329-3440
d P6 nt283-418Δ3329-3440
7 3' RACE F nt3518-d(T)18V a (pA)d at nt 5149
b (pA)p at nt 3771
c non-specific
*

Nucleotide numbers shown refers to Genbank accession no.: NC_007018.

“Δ” denotes intron.