Table 1.
Lane | Analysis | Primers* | Bands | Identified transcripts* |
---|---|---|---|---|
1 | 5' RACE | d(G)16-R nt960 | a | P6 nt262-960 |
2 | 5' RACE | d(G)16-R nt3840 | a | P38 nt1950-2060Δ2309-2367Δ3329-3840 |
b | P38 nt1950-2013Δ3329-3840 | |||
3 | RT-PCR | F nt283-R nt2060 | a | P6 nt283-2060 |
b | P6 nt283-418Δ1630-2060 | |||
4 | RT-PCR | F nt1441-R nt2354 | a | non-specific |
b | P6 nt1441-2354 | |||
c | P6 nt1441-2060Δ2309-2354 | |||
5 | 5' RACE | d(T)18V-R nt3440 | a | P38 nt1950-2060Δ2309-2367Δ3329-3440 |
b | P38 nt1950-2013Δ3329-3440 | |||
c | Primer dimers | |||
6 | RT-PCR | F nt283-R nt3440 | a | P6 nt283-2060Δ2309-2367Δ3329-3440 |
b | P6 nt283-418Δ1630-2060Δ2309-2367Δ3329-3440 | |||
c | P6 nt283-418Δ1630-2013Δ3329-3440 | |||
d | P6 nt283-418Δ3329-3440 | |||
7 | 3' RACE | F nt3518-d(T)18V | a | (pA)d at nt 5149 |
b | (pA)p at nt 3771 | |||
c | non-specific |
Nucleotide numbers shown refers to Genbank accession no.: NC_007018.
“Δ” denotes intron.