Fig 5.

Biodistributions of 111In-labeled biotin compounds in tumors and normal organs of athymic mice bearing Ramos xenografts pretargeted with 1F5-S45A-SAv, 1F5-Y43ASAv, or 1F5-WT-SAv FP. Mice were fed either normal or biotin-deficient diets for 5-6 days before injection of 2.8 nmol of FPs, as indicated. Twenty-four hours later 5.8 nmol of either monovalent NAGB CA or divalent bis-biotin CA was injected. After another 4 hours, mice were injected with 1.2 nmol of 111In-labeled DOTA-biotin or 111In-labeled bisbiotin. Results are shown for mice sacrificed either 24 hours (A) or 48 hours (B) after administration of the radiolabeled biotin ligands.