Figure 6.

Targeting MLL1 reduces GSC-mediated VEGF production and endothelial cell proliferation. Following puromycin selection of infected GSCs isolated from (a) 4121 or (b) 387 xenografts, fresh GSC cell culture media without growth factors were added for 48 h. Conditioned media were passaged through a 0.22 μm syringe filter and then used to measure VEGF production by ELISA. (c) Following a similar media collection as detailed above and as shown in the diagram on the left, HUVECs were grown in the conditioned media for 24 h before the addition of tritiated thymidine for 4 h. Thymidine incorporation was measured using a scintillation counter. ^*P<0.001; ^**P<0.05