Figure 8.

MLL1 inhibition reduces tumor propagation in mice. (a) In all, 387 GSCs bearing stable NT or MLL1 shRNA tagged with GFP was implanted into the right frontal lobe of athymic nu/nu mice. Mice were monitored for signs of neurological deficits. ^*P<0.01. (b) Following euthanasia, whole mouse brains were fixed and frozen. A total of 10 μM sections were stained for GFP to assess the presence of cells transduced with NT or MLL1 shRNAs. Tumors from shRNA-bearing mice (n=3 per group) contained fewer GFP-positive cells, indicating that tumors forming in the cohort were likely from an contaminating GFP-negative cells