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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Allergy Clin Immunol. 2011 Aug 6;128(6):1318–1325.e2. doi: 10.1016/j.jaci.2011.06.041

Fig 5.

Fig 5

SRWe induces TSLP mRNA and protein by HCECs through TLR4 and NF-κB signaling pathways. A, B. Primary HCECs were treated with 0.1 to 50 μg/ml of SRWe for 4 hours for TSLP mRNA or 48 hours for TSLP protein in the supernatants. C, D. HCECs were pre-incubated with mouse TLR4 antibody (10μg/ml), isotype mouse IgG2a k, or NF-kB Activation Inhibitor quinazoline (NFkB-I, 10μM) for 1 hour before adding 10μg/ml SRWe for 4 hours for TSLP mRNA or 48 hours for TSLP protein in the supernatants. Results shown are the Mean ± SD (n=4). *P<0.05, **P<0.01.