Table 1.
Details of primary antisera used for immunohistochemistry
| Antibody | Source | Retrieval | Species | Dilution |
|---|---|---|---|---|
| BrdU | Fitzgerald Industries, MA | Yes | Sheep | 1 : 1000 |
| DAZL | AbD Serotec, Oxford | Yes | Mouse | 1 : 300 |
| DMRT1 | Gift from David Zarkower | Yes | Rabbit | 1 : 2000 |
| OCT4 | Santa Cruz, CA, USA | Yes | Goat | 1 : 100 |
| SMA | Novocastra, Newcastle | No | Mouse | 1 : 1000 |
| VASA | Abcam, Cambridge | Yes | Rabbit | 1 : 200 |
BrdU, bromo-2′-deoxyuridine-5′-monophosphate; DMRT1, Doublesex and MAB-3-related transcription factor 1; OCT4, octamer-binding transcription factor 3/4; SMA, smooth muscle actin.