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Genes predicted to be essential by FBA and by two independent transposon mutageneses. The essential genes determined by FBA include "metabolic genes" only, shown in green. The "non-metabolic genes" are indicated in red. The genes predicted to be essential by transposon mutagenesis were those with no insert in the transposon mutagenesis in this study and in the study of Stahl and Stintzi [20].
