Table 3.
List of essential "metabolic genes" according to the different techniques
| Technique | Essential genes |
|---|---|
| FBA only | cj0024, cj0026c, cj0066c, cj0075c, cj0127c, cj0146c, cj0172c, cj0187c, cj0194, cj0196c, cj0197c, cj0205, cj0227, cj0237, cj0240c, cj0273, cj0274, cj0286c, cj0288c, cj0296c, cj0297c, cj0298c, cj0306c, cj0307, cj0321, cj0326, cj0332c, cj0360, cj0384c, cj0405, cj0432c, cj0433c, cj0434, cj0435, cj0437, cj0443, cj0453, cj0490, cj0541, cj0542, cj0559, cj0576, cj0580c, cj0585, cj0589, cj0638c, cj0647, cj0699c, cj0716, cj0764c, cj0766c, cj0767c, cj0795c, cj0798c, cj0806, cj0813, cj0821, cj0822, cj0847, cj0853c, cj0858c, cj0862c, cj0891c, cj0905c, cj0918c, cj0932c, cj0947c, cj0949c, cj0955c, cj0992c, cj0995c, cj1039, cj1044c, cj1046c, cj1048c, cj1067, cj1080c, cj1081c, cj1088c, cj1096c, cj1104, cj1114c, cj1133, cj1149c, cj1150c, cj1151c, cj1152c, cj1177c, cj1183c, cj1196c, cj1198, cj1202, cj1213c, cj1238, cj1243, cj1248, cj1364c, cj1398, cj1400c, cj1401c, cj1402c, cj1404, cj1407c, cj1424c, cj1428c, cj1476c, cj1498c, cj1515c, cj1529c, cj1530, cj1605c, cj1634c, cj1641, cj1645, cj1672c, cj1685c |
| FBA & transposon mutagenesis of this study | cj0027, cj0116, cj0117, cj0324, cj0387, cj0394c, cj0442, cj0514, cj0516, cj0581, cj0597, cj0639c, cj0641, cj0686, cj0840c, cj0894c, cj0927, cj1008c, cj1045c, cj1054c, cj1058c, cj1082c, cj1131c, cj1250, cj1274c, cj1288c, cj1346c, cj1347c, cj1366c, cj1403c, cj1443c, cj1536c, cj1607, cj1610, cj1652c |
| FBA & transposon mutagenesis of Stahl and Stintzi | cj0132, cj0147c, cj0308c, cj0356c, cj0451, cj0503c, cj0722c, cj0845c, cj0861c, cj0925, cj1201, cj1239, cj1290c, cj1291c, cj1531, cj1535c |
| FBA & the 2 transposon mutagenesis methods | cj0231c, cj0545, cj0707, cj0810, cj0855, cj0895c, cj1644, cj1676 |
The "metabolic genes" refer to the genes that were included in the FBA model. This table gives the list of the genes shown in Figure 3. The genes predicted to be essential by transposon mutagenesis were those with no insert in the transposon mutagenesis in this study and in the study of Stahl and Stintzi [20].