Table 1.

Experiment I. Anti-Bm86 levels in mice immunized according to the experimental groups^a.

	Days
Immunogen / adjuvant	10	20	30	40
Bm86 / Montanide 888	0.23 ± 0.24	1.34 ± 0.58	2.17 ± 0.54	1.02 ± 0.12
Bm86 / cells	0.17 ± 0.05	0.16 ± 0.10	0.24 ± 0.31	0.25 ± 0.07
Bm86 / membrane	0.17 ± 0.07	1.20 ± 0.67	2.09 ± 0.48	1.09 ± 0.27
Bm86 / membrane / saponin	0.23 ± 0.09	2.16 ± 0.39	2.68 ± 0.18	1.09 ± 0.12
Cells	0.15 ± 0.07	0.07 ± 0.05	0.18 ± 0.06	0.19 ± 0.05
Membrane	0.22 ± 0.08	0.19 ± 0.11	0.26 ± 0.11	0.26 ± 0.14
Membrane / saponin	0.21 ± 0.06	0.12 ± 0.03	0.23 ± 0.08	0.25 ± 0.11

^a Ten mice per group were immunized with 5 μg Bm86 adjuvated as indicated (Garcia - Garcia et al., 1995). ELISA was used to determine total antibody response against Bm86.Briefly, 100 μl of antiserum diluted 1: 100 in PBS were added to the wells, incubated for 2 h at 37°C, washed four time with PBST and developed with HRP-conjugated goat anti-mouse IgG as describe in material and methods. Antibody titres were expressed as the mean absorbance value (O.D._492nm) per group ± SD (N = 10).