Table 3.
IgG1/IgG2a | ||||
Immunogen / adjuvant | IgG1 | IgG2a | IgG2b | |
Ratio | ||||
Bm86 / Montanide 888 | 2000 | 200 | 100 | 10 |
Bm86 / cells | 32 | 4 | 8 | 8 |
Bm86 / membrane | 400 | <50 | <50 | >8 |
Bm86 / membrane / saponin | 1000 | 400 | 100 | 2.5 |
a Experiments were conducted as described in Table 2. The IgG samples were homogenized to the same protein concentration and specific anti-Bm86 antibody levels were determined by ELISA and expressed as antibodies titres (Garcia - Garcia et al.,1995).