FIG. 3.

Different cellular characteristics including (A) BrdU labeling, cell in (B) G0/G1, and (C) G2/M stage, and (D) apoptotic cells in control and bovine fibroblast cells treated with various concentrations of trichostatin A (TSA) (0. 0.03, 0.08, and 1 μM), sodium butyric (NaB) (0, 0.5, 1, and 2 mM) and 5-aza-2′-deoxycytidine (AzC) (0, 0.01, 0.08, and 0.3 μM) for 24, 96, and 72 h, respectively (black columns), and after drug removal for 24 h (white columns). Comparisons were carried out at three levels: (1) within the drug-treated groups, (2) within the drug removal groups, and (3) between drug-treated and drug-removal groups. Accordingly, values with at least one common letter on black or white columns indicate nonsignificant difference within the drug treated and drug removal groups, whereas asterisks indicate significant difference between drug treatment and drug removal for each drug treatment at each concentration at p<0.05.