FIG. 4.

(A) Immunostaining of BEF cells treated with TSA and NaB for Ac-H3K9 and cells treated with AzC for 5-MC. Cells were immunostained with anti-H3K9 or anti-5MC (grey). In nontreated cells intensity of Ac-H3K9 was weak, whereas in TSA- and NaB-treated cells intensity was strong. On the other hand, 5-MC intensity is also strong in nontreated cells compared with AzC-treated cells. Scale bar represents 200 μm. Relative levels of (B) DNA methylation and (C) histone H3K9 acetylation in control and treated bovine fibroblast cells treated with various concentrations of trichostatin A (TSA), sodium butyric (NaB), and 5-aza-2′-deoxycytidine (AzC) for 24, 96, and 72 h, respectively (black column) and after drug removal for 24 h (white column). Comparison was carried out between drug groups or the drug removal groups. Different subscript indicate significantly difference between the groups, whereas asterisks indicate significant difference between drug treatment and drug removal for each drug treatment at each concentration at p<0.05.