Figure 3.

Effect of ethylene gas on [Ca²⁺]_i level in SaOS-2 cells kept under pressure. Cells grown on coverslip were loaded with Fura-2 and transferred into a pressure chamber as described in Materials and Methods. A. Cells inspected by Nomarski phase contrast interference optics. B. Untreated cells. C. Cells kept under pressure (1 atm) for 10 min. D. Cells kept under pressure (1 atm) 10 min after ethylene gas injection into the pressure chamber. Magnification, 200-fold. For further details, see Figure 1.