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. 2011 Oct 23;3(10):1003–1017. doi: 10.18632/aging.100394

Figure 2. GAPDH pool levels in plantaris/FHL lysate from old mice.

Figure 2

Protein (30 μg) from total muscle cell lysates of plantaris/FHL from old mice (24-27 months) subjected to two hours of ischemia were separated by SDS-PAGE, transferred to PVDF membrane, and blotted with anti-GAPDH antibody. (A) Groups I, one day; II, 3 days, III, 5 days and IV, 7 days of reperfusion all showed statistically significant decreases in GAPDH immunoreactive pool levels. (B) A bar graph showing the statistical differences in GAPDH pool levels in response to 2 hours of ischemia and 1-7 days of reperfusion.