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. 2011 Oct 23;3(10):1003–1017. doi: 10.18632/aging.100394

Table 1. Identification of 3-Nitrotyrosine Modified Proteins by Mass Spectrometry.

# Protein Name (Mus Musculus) Protein MW Peptide Count Protein Score Protein PI Expectation Score
Young - 5 Days Reperfusion
1 Aconitate hydratase, mitochondrial precursor 85,410.0 13 70 8.08 1.26E-03
2 Actin, alpha cardiac muscle 1 41,991.9 10 450 5.23 1.26E-41
3 Actin, alpha skeletal muscle 42,023.9 12 482 5.23 7.92E-45
4 Actin, cytoplasmic 2 41,765.8 7 220 5.31 1.26E-18
5 Actin, gamma-enteric smooth muscle 41,849.8 8 304 5.31 5.00E-27
6 Annexin A11 54,076.8 11 146 7.53 3.15E-11
7 ATP synthase subunit alpha, mitochondrial precursor 59,715.6 17 290 9.22 1.26E-25
8 Carbonic anhydrase 29,347.6 5 57 6.89 2.51E-02
9 Carbonic anhydrase 3 29,347.6 8 183 6.89 6.29E-15
10 Creatine kinase M-type 43,017.8 16 562 6.58 7.92E-53
11 Desmin 53,456.0 12 67 5.21 2.51E-03
12 Dihydrolipoyl dehydrogenase, mitochondrial precursor 54,238.2 10 269 7.99 1.58E-23
13 ETS-related transcription factor Elf-3 44,245.6 10 59 5.75 1.58E-02
14 Fructose-bisphosphate aldolase A 39,331.3 10 108 8.31 1.99E-07
15 Glutamate dehydrogenase 1, mitocondrial precursor 61,298.2 8 75 8.05 3.97E-04
16 Glyceraldehyde-3-phosphate dehydrogenase 35,787.2 9 180 8.44 1.26E-14
17 Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic 37,548.4 13 311 6.75 9.98E-28
18 Heat shock protein beta-1 22,999.7 8 254 6.12 5.00E-22
19 Malate dehydrogenase, cytoplasmic 36,488.1 7 188 6.16 1.99E-15
20 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial precursor 27,267.9 4 54 7 5.00E-02
21 Nebulin-related-anchoring protein 195,651.6 23 59 9.35 1.58E-02
22 Peroxiredoxin-6 24,855.0 5 149 5.71 1.58E-11
23 Pyruvate kinase isozymes M1/M2 57,808.0 13 128 7.18 1.99E-09
24 Rho GDP-dissociation inhibitor 1 23,392.8 11 270 5.12 1.26E-23
25 Serum albumin precursor 68,647.7 5 207 5.75 2.51E-17
26 Triosephosphate isomerase 26,695.8 6 199 6.9 1.58E-16
27 UTP-glucose-1-phosphate uridylyltransferase 56,943.7 11 67 7.18 2.51E-03
Old - 5 Days Reperfusion
# Protein Name (Mus Musculus) Protein MW Peptide Count Protein Score Protein PI Expectation Score
1 Actin, alpha cardiac muscle 1 41,991.9 4 98 5.23 1.99E-06
2 Actin, alpha skeletal muscle 42,023.9 4 97 5.23 2.51E-06
3 Actin, cytoplasmic 1 41,709.7 8 134 5.29 5.00E-10
4 Actin, cytoplasmic 2 41,765.8 2 57 5.31 2.51E-02
5 Actin, gamma-enteric smooth muscle 41,849.8 3 63 5.31 6.29E-04
6 Ankyrin repeat domain-containing protein 49 27,094.5 7 54 5.04 5.00E-02
7 Annexin A11 54,076.8 10 299 7.53 1.58E-26
8 Apolipoprotein A-1 precursor 30,568.7 17 622 5.64 7.92E-59
9 ATP synthase subunit alpha, mitochondrial precursor 59,715.6 16 383 9.22 6.29E-35
10 Creatine kinase M-type 43,017.8 13 456 6.58 3.15E-42
11 Fibrinogen beta chain precursor 54,717.7 8 90 6.68 1.26E-05
12 Fructose-bisphosphate aldolase A 39,331.3 5 68 8.31 1.99E-03
13 Glyceraldehyde-3-phosphate dehydrogenase 35,787.2 5 150 8.44 1.26E-11
14 Ig gamma-2B chain C region membrane-bound form 44,231.2 3 174 6.1 3.00E-14
15 Ig gamma-2B chain C region secreted form 36,635.3 3 175 7.19 3.97E-14
16 Ig kappa chain C region 11,770.5 2 68 5.23 1.99E-03
17 Lamin-A/C 74,192.7 7 72 6.54 7.92E-04
18 L-lactate dehydrogenase A chain 36,475.2 7 117 7.62 2.51E-08
19 Myosin-1 223,203.0 8 161 5.6 9.98E-13
20 Myosin-3 223,652.4 8 121 5.62 9.98E-09
21 Myosin-4 222,719.9 9 264 5.58 5.00E-23
22 Myosin-6 223,426.5 24 441 5.57 9.98E-41
23 Myosin-7 222,740.5 28 537 5.59 2.51E-50
24 Myosin-8 222,569.5 10 231 5.65 9.98E-20
25 Nucleoside diphosphate kinase B 17,351.9 4 60 6.97 1.26E-02
26 Peptidyl-prolyl cis-trans isomerase A 17,959.8 2 55 7.74 3.97E-02
27 Phosphoglycerate mutase 1 28,813.9 8 175 6.67 3.97E-14
28 Phosphoglycerate mutase 2 28,808.8 4 103 8.65 6.29E-07
29 Putative RNA-binding protein 3 16,594.7 8 258 6.84 1.99E-22
30 Serotransferrin precursor 76,673.7 13 294 6.94 5.00E-26
31 Serum albumin precursor 68,647.7 5 124 5.75 5.00E-09
32 Strumpellin 134,024.8 12 57 6.72 2.51E-02
28 Superoxide dismutase [Cu-Zn] 15,932.8 9 406 6.02 3.15E-37
29 Transthyretin precursor 15,766.0 9 446 5.77 3.15E-41
30 Triosephosphate isomerase 26,695.8 6 246 6.9 3.15E-21
31 Tropomyosin alpha-1 chain 32,660.7 26 687 4.69 2.51E-65
32 Tropomyosin alpha-3 chain 32,842.8 13 206 4.68 3.15E-17
33 Tropomyosin alpha-4 chain 28,450.4 6 68 4.65 1.99E-03
34 Tropomyosin beta chain 32,816.6 18 275 4.66 3.97E-24