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. 2011 Oct 28;3(10):1028–1038. doi: 10.18632/aging.100397

Figure 5. Effect of metformin on constitutive expression γH2AX and ATM-S1981P in normal human proliferating lymphocytes.

Figure 5

Peripheral blood lymphocytes were mitogenically stimulated by phytohemagglutinin for 48 h and then were grown in the absence (Ctrl) or presence of 5 mM metformin (MF) for additional 24 h. The expression of γH2AX and ATM-S1981P was detected with phospho-specific Abs and cell fluorescence we as measured by flow cytometry. The numerical figures show the percent reduction in expression of γH2AX and ATM-S1981P of cells treated with metformin with respect to Ctrl, in the respective phases of the cell cycle.