Fig. 5.

Protein analysis of yeast lipid particles and quality control. A, Protein patterns of the lipid particle fraction from Saccharomyces cerevisiae grown on glucose or oleate. Low molecular weight standards are shown in the lane on the left. Lanes were loaded with 15 μg total protein, each. Proteins were stained with Coomassie Blue. B, Quality control of subcellular fractions. Western blot analysis was performed with homogenate (H), lipid particle (L) and an organelle pellet fraction (P) obtained as side product of LP isolation from cells grown on glucose or oleate. Antisera used were reactive with Erg1p, squalene monooxygenase (LP marker); Pma1p, plasma membrane H⁺-ATPase (plasma membrane marker); Por1p, mitochondrial porin (mitochondrial marker); Pcs60p, peroxisomal AMP-binding protein (peroxisomal marker); Fox1p, peroxisomal fatty acyl CoA oxidase (peroxisomal marker); and Wbp1p, beta subunit of the oligosaccharyl transferase glycoprotein complex (microsomal marker).