Fig. 7.

Subcellular localization of newly discovered lipid particle proteins. Cell fractionation was performed with C-terminally tagged proteins expressed under native promoters. Cells were cultivated on glucose or oleate, respectively, and subcellular fractions were prepared as described in the Methods and materials section. H, homogenate; P, organelle pellet fraction obtained as side product of LP isolation; L, lipid particles; ER, endoplasmic reticulum (30,000 g and 40,000 g microsomes); C, cytosol; Px, peroxisomes; Mit, mitochondria.