Table 6.
Proteome of yeast lipid particles. §novel LP protein, found in cells grown on glucose or oleate, ¥ numbers indicate fragments used for identification of proteins from cells grown on glucose (D) or oleate (O). Proteins identified by only single peptides were excluded. C, cytosol; M, mitochondria; PM, plasma membrane; ER, endoplasmic reticulum; LP, lipid particle; End, endosomes; G, Golgi; Mic, microsomes; V, vacuole; Px, peroxisome; N, nucleus; nEnv, nuclear envelope; ext, extrinsic to membrane; mem, integral to membrane; bud, cellular bud; rib, ribosomal subunit; CW, cell wall; R, ribosome; Retro, retrotransposon; mTub, microtubule. Databases used: SGD (http://www.yeastgenome.org/20112010); GFP (http://yeastgfp.yeastgenome.org/20112010); YPL (http://ypl.tugraz.at/20112010).
| Gene name | Systematic name | SGD | GFP | YPL | This study | D¥ | O¥ | Localization (SGD) | Description |
|---|---|---|---|---|---|---|---|---|---|
| ACH1 | YBL015W | ✓ | 2 | C/M | CoA transferase activity | ||||
| ADH1 | YOL086C | ✓ | 2 | C/PM | Alcohol dehydrogenase | ||||
| ALG9 | YNL219C | ✓ | 2 | ER | Mannosyltransferase | ||||
| ATF1 | YOR377W | ✓ | LP/End | Alcohol acyltransferase | |||||
| ATP2 | YJR121W | ✓ | 9 | M | Subunit of mitochondria ATP synthase | ||||
| AYR1 | YIL124W | ✓ | ✓ | ✓ | 15 | 12 | C/ER/LP/M | NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase | |
| BSC2 | YDR275W | ✓ | ✓ | LP | Unknown | ||||
| COY1 | YKL179C | ✓ | G | Golgi membrane protein | |||||
| CPR5§ | YDR304C | ✓ | 7 | 3 | C/ER | Peptidyl-prolyl cis-trans isomerase | |||
| CSR1 | YLR380W | ✓ | LP/C/M/Mic | Phosphatidylinositol transfer protein | |||||
| CST26 | YBR042C | ✓ | ✓ | ✓ | LP | Required for incorporation of stearic acid into phosphatidylinositol | |||
| CWH43 | YCR017C | ✓ | 2 | PM | Putative sensor/transporter protein | ||||
| DFM1 | YDR411C | ✓ | 2 | ER | ER localized derlin-like family member | ||||
| DGA1 | YOR245C | ✓ | ✓ | 3 | LP | Diacylglycerol acyltransferase | |||
| DPL1 | YDR294C | ✓ | 2 | ER | Dihydrosphingosine phosphate lyase | ||||
| DPM1 | YPR183W | ✓ | 2 | ER/M | Dolichol phosphate mannose synthase | ||||
| EHT1 | YBR177C | ✓ | ✓ | ✓ | 19 | 18 | LP/M | Acyl-coenzymeA:ethanol O-acyltransferase | |
| ENO2 | YHR174W | ✓ | 2 | V/PM/M | Enolase II | ||||
| ERG1 | YGR175C | ✓ | ✓ | ✓ | 5 | 15 | ER/LP | Squalene epoxidase | |
| ERG6 | YML008C | ✓ | ✓ | ✓ | ✓ | 37 | 30 | ER/LP/M | Delta(24)-sterol C-methyltransferase |
| ERG7 | YHR072W | ✓ | ✓ | ✓ | ✓ | 7 | 2 | ER/LP/PM | Lanosterol synthase |
| ERG27 | YLR100W | ✓ | ✓ | ✓ | ✓ | 9 | 4 | ER/M | 3-Keto sterol reductase |
| FAA1 | YOR317W | ✓ | ✓ | 20 | 15 | LP/PM/M | Long chain fatty acyl-CoA synthetase | ||
| FAA3 | YIL009W | ✓ | 3 | unknown | Long chain fatty acyl-CoA synthetase | ||||
| FAA4 | YMR246W | ✓ | ✓ | ✓ | ✓ | 13 | 6 | LP/C | Long chain fatty acyl-CoA synthetase |
| FAS1 | YKL182W | ✓ | LP/C/M | Fatty acid synthetase | |||||
| FAT1 | YBR041W | ✓ | ✓ | ✓ | ✓ | 13 | 9 | PM/LP/Mic/PX | Fatty acid transporter |
| FMP52 | YER004W | ✓ | 4 | ER/M | Unknown | ||||
| GPT2 | YKR067W | ✓ | 12 | C/ER | sn-1 Acyltransferase | ||||
| GTT1§ | YIR038C | ✓ | 2 | 8 | ER/M/PM | Glutathione S-transferase | |||
| GVP36 | YIL041W | ✓ | 3 | C/G | BAR domain-containing protein | ||||
| HFD1 | YMR110C | ✓ | ✓ | ✓ | 7 | 18 | M/LP/End | Putative fatty aldehyde dehydrogenase | |
| HSP12 | YFL014W | ✓ | 6 | C/PM/N | Heat shock protein | ||||
| KAR2 | YJL034W | ✓ | 9 | ER | ATPase | ||||
| LAP4 | YKL103C | ✓ | 3 | V | Vacuolar aminopeptidase | ||||
| LDB16 | YCL005W | ✓ | ✓ | LP/M | Unknown | ||||
| MSC1 | YML128C | ✓ | 2 | M/ER/PM | Unknown | ||||
| NUS1 | YDL193W | ✓ | ✓ | ✓ | 9 | 4 | ER/LP/nEnv | Putative prenyltransferase | |
| OSH4§ | YPL145C | ✓ | 3 | 5 | C/G/ext | Oxysterol binding protein | |||
| OSW5 | YMR148W | ✓ | ✓ | 2 | mem | Unknown | |||
| PDI1§ | YCL043C | ✓ | 6 | 7 | ER | Disulfide isomerase | |||
| PDR16 | YNL231C | ✓ | ✓ | ✓ | 5 | 9 | LP/Mic/PM/C | Phosphatidylinositol transfer protein | |
| PET10 | YKR046C | ✓ | ✓ | ✓ | ✓ | 12 | 24 | LP | Unknown |
| PGC1 | YPL206C | ✓ | ✓ | 9 | LP/M | Phosphatidylglycerol phospholipase C | |||
| PIL1 | YGR086C | ✓ | C/M/PM | Primary component of eisosomes | |||||
| PMA1 | YGL008C | ✓ | 3 | PM/M/mem | Plasma membrane H + − ATPase | ||||
| PMT1 | YDL095W | ✓ | 2 | ER | Protein O-mannosyltransferase | ||||
| PMT2 | YAL023C | ✓ | 3 | ER | Protein O-mannosyltransferase | ||||
| POR1 | YNL055C | ✓ | 2 | M | Mitochondrial porin | ||||
| POX1 | YGL205W | ✓ | 3 | PX | Fatty-acyl coenzyme A oxidase | ||||
| RHO1 | YPR165W | ✓ | 4 | mem/PX/PM/M/bud | GTP-binding protein | ||||
| RPL5 | YPL131W | ✓ | 3 | rib | Protein component of the large (60S) ribosomal subunit | ||||
| RPL10 | YLR075W | ✓ | 2 | rib | Protein component of the large (60S) ribosomal subunit | ||||
| RPS1B | YML063W | ✓ | 2 | rib | Ribosomal protein 10 (rp10) of the small (40S) subunit | ||||
| RPS3 | YNL178W | ✓ | 5 | rib | Protein component of the small (40S) ribosomal subunit | ||||
| RPS19B | YNL302C | ✓ | 2 | rib | Protein component of the small (40S) ribosomal subunit | ||||
| RPS31 | YLR167W | ✓ | 4 | rib/C | Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin | ||||
| RRT8 | YOL048C | ✓ | ✓ | ✓ | 4 | 2 | LP | Unknown | |
| RTN2 | YDL204W | ✓ | 3 | ER/nEnv | Unknown | ||||
| SEC61 | YLR378C | ✓ | 5 | ER | Essential subunit of Sec61 complex | ||||
| SEC63 | YOR254C | ✓ | 4 | ER/M | Essential subunit of Sec63 complex | ||||
| SHE10 | YGL228W | ✓ | 4 | unknown | Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function | ||||
| SLC1 | YDL052C | ✓ | ✓ | ✓ | 2 | 3 | LP | 1-Acyl-sn-glycerol-3-phosphate acyltransferase | |
| SNA2 | YDR525W-a | ✓ | ✓ | Mem/C | Unknown | ||||
| SNX41 | YDR425W | ✓ | End | Sorting nexin | |||||
| SRT1 | YMR101C | ✓ | LP | Cis-prenyltransferase | |||||
| SSA1 | YAL005C | ✓ | 2 | C/PM/N | ATPase | ||||
| SSO1 | YPL232W | ✓ | PM | Plasma membrane t-snare | |||||
| TDH1 | YJL052W | ✓ | ✓ | 4 | C/LP/M/PM/CW | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1 | |||
| TDH2 | YJR009C | ✓ | C/LP/M/PM/CW | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2 | |||||
| TDH3 | YGR192C | ✓ | ✓ | 5 | C/LP/M/PM/CW | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3 | |||
| TEF1 | YPR080W | ✓ | 5 | M/R | Translational elongation factor EF-1 alpha | ||||
| TGL1 | YKL140W | ✓ | ✓ | 12 | 14 | LP/mem | Steryl ester hydrolase | ||
| TGL3 | YMR313C | ✓ | ✓ | ✓ | 4 | 5 | LP | Triacylglycerol lipase | |
| TGL4 | YKR089C | ✓ | ✓ | ✓ | 3 | 4 | LP | Triacylglycerol lipase | |
| TGL5 | YOR081C | ✓ | ✓ | ✓ | 5 | 3 | LP | Triacylglycerol lipase | |
| USE1 | YGL098W | ✓ | ER | SNARE protein | |||||
| TSC10 | YBR265W | ✓ | 2 | C/ER/M | 3-ketosphinganine reductase | ||||
| TUB2 | YFL037W | ✓ | 2 | mTub | Beta-tubulin | ||||
| UBX2§ | YML013W | ✓ | 5 | 3 | ER/M | Protein involved in ER-associated protein degradation | |||
| VPS66§ | YPR139C | ✓ | 6 | 4 | C | Cytoplasmic protein of unknown function involved in vacuolar protein sorting | |||
| WBP1 | YEL002C | ✓ | 3 | ER/nEnv | Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex | ||||
| YBR204C | YBR204C | ✓ | ✓ | 3 | unknown | Serine hydrolase | |||
| YDR018C | YDR018C | ✓ | unknown | Similarity to acyltransferase | |||||
| YEH1 | YLL012W | ✓ | ✓ | LP/mem | Steryl ester hydrolase | ||||
| YIM1 | YMR152W | ✓ | ✓ | ✓ | 4 | LP/C/M | Unknown | ||
| YGR038C-B | YGR038C-B | ✓ | 2 | Retro | Retrotransposon TYA Gag and TYB Pol genes | ||||
| YJU3 | YKL094W | ✓ | ✓ | ✓ | 3 | 2 | LP/C/M/PM | Serine hydrolase | |
| YNL134C | YNL134C | ✓ | 2 | C/N | Unknown | ||||
| YNL208W | YNL208W | ✓ | 3 | M/R | Unknown | ||||
| YOR059C | YOR059C | ✓ | LP | Unknown | |||||
| YOR246C | YOR246C | ✓ | ✓ | LP | Similarity to oxidoreductase | ||||
| YPT7§ | YML001W | ✓ | 4 | 2 | V/M | GTPase | |||
| ZEO1 | YOL109W | ✓ | 9 | M/PM/ext | Peripheral membrane protein |