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Colocalization of POLβ and PNK with XRCC1 in replication foci. (A–D) Cotransfection of HeLa cells expressing XRCC1-EYFP with ECFP-POLβ or (E–H) ECFP-PNK together with HcRed-tagged PCNA as a replication marker. White spots (merged) demonstrate colocalization of POLβ, XRCC1, PCNA (D) and PNK, XRCC1, PCNA (H). Yellow spots show colocalization of POLβ and XRCC1 (D) and PNK and XRCC1 (H). Bars, 5 μm.
