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. 2011 Dec 5;2:239. doi: 10.3389/fmicb.2011.00239

Figure 2.

Figure 2

(A) Overview of the strategy used for purification of CMHs from fungal cells. (B) High-performance thin-layer chromatography of fungal neutral glycosphingolipids isolated by silica gel column chromatography. Lane 1: cerebroside from bovine brain. Lanes 2–6: Chloroform–methanol (95:5 v/v) fractions. Lanes 7–8: Chloroform–methanol (90:10 v/v) fractions. Lanes 9–11: Chloroform–methanol (80:20 v/v) fractions. Solvent system: chloroform/methanol ½ M NH4OH (40:10:1 v/v/v). Detection: iodine and orcinol–sulfuric acid reagents.