Figure 7.

Two-dimensional NMR fingerprints of F. oxysporum CMH observed by homonuclear and heteronuclear experiments. (A) Overlaid representation of fingerprint assignments of partial edited HSQC and HMBC spectra; positive phase (blue) correspond to CH and CH3 carbons, negative phase (green) correspond to CH2 carbons, in (red) HMBC cross peaks; (boxes) represent glycosyl-ceramide linkage zones fingerprints. (B) Partial TOCSY (blue) and COZY (red) spectra from the anomeric region showing the main cross peaks of the carbohydrate ring, observation of the total ¹H–¹¹H axial correlations and reach the monosaccharide composition. (C–E) HSQC and HMBC slices of unsaturated carbons in ceramide moiety. (E,F) Fingerprint slices of the quaternary carbons (C-9) and amide linkage at 136.4 and 176.5 ppm, respectively.