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. 2011 Dec 1;5:79. doi: 10.3389/fnint.2011.00079

Figure 2.

Figure 2

(A) Representative agarose gel blot analysis for human GAPDH gene of 30 days-SNI/hMSCs mice following RT-PCR is shown. Lane (1) lung; lane (2) sciatic nerve; lane (3) ventral L4–L5 spinal cord and (4) dorsal L4–L5 spinal cord; lane (5) pre-frontal cortex; lane (6) DNA ladder marker. The analysis of mRNA levels was carried out by the “Gel Doc 2000 UV System” (Bio-Rad, Hercules, CA, USA). RT-PCR analysis revealed a preferential accumulation of hMSCs to the L4–L5 spinal cord (both ventral and dorsal areas) and pre-frontal cortex. Very weak band was found from lung. No GAPDH band was detectable in sciatic nerve, thus revealing the specificity of hMSC engraftment at the NP controlling and processing areas. (B) Representative cross-section of mouse whole L4–L5 spinal cord area from hMSC-treated 30 days-neuropathic mice (26 days post-hMSC injection). (B’) Representative cross-section of mouse whole L4–L5 spinal cord area from vehicle treated 30 days-neuropathic mice showing no staining for human specific CD73. (C,D) Human MSCs expressed lineage-specific antigens at 26 days post-injection in vivo. Representative fluorescent photomicrograph of hMSCs showing immunocytochemistry for CD73. Area in white rectangle insets is shown: (C) ventral spinal cord; (D) dorsal spinal cord; left: cell nuclei were counterstained with DAPI (blue fluorescence); middle: CD73-positive hMSCs emitted red fluorescence; right: pictures with both red and blue fluorescence were merged. Scale bars: 100 μm.