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. 2011 Oct 25;152(12):4894–4905. doi: 10.1210/en.2011-1492

Fig. 5.

Fig. 5.

A–C, Representative photomicrographs from a rat receiving a terminal microinfusion of fluorescent dextran (red). These photomicrographs were created by combining brightfield images with fluorescent images of the red dextran dye. The numbers in the upper right corner of each photomicrograph indicate the plate number from the Paxinos rat brain atlas (42) and distance from Bregma. Diffusion of fluorescent dextran was identified in the vertical limb of the diagonal band of Broca (A), the medial septal nucleus (A and B), septohypothalamic nucleus (B and C), and the MnPO (B and C). The guide cannula tract and a dense deposit of fluorescent dextran is identified in A. D and E, Representative photomicrographs of Fos immunohistochemistry in OVX + E2 rats killed 90 min after microinfusion of either vehicle (D) or senktide (E) at the ambient temperature of 21.5 C. Microinfusion of senktide dramatically increased the number of Fos-ir cells in the MnPO (E). 3V, Third ventricle; ac, anterior commissure, LV, lateral ventricle; MS, medial septum, POA, preoptic area; SHy, septohypothalamic nucleus; oc, optic chiasm; VDB, vertical limb of the diagonal band of Broca. Scale bars, 500 μm (A–C) and 100 μm (D and E).