Fig. 1.

Sex-dependent hormonally regulated expression levels of CYP3A4 protein in hepatocytes derived from adult men and women. The cells were exposed to either continuous dexamethasone alone (DEX), continuous GH alone, both hormones (DEX+GH), or vehicle alone (-DEX-GH) for 5 d in culture, after which the cells were harvested and analyzed. Each data point is a mean ± sd for cells from six men, all treatments except DEX+GH, in which n = 5; six women, all treatments except -DEX-GH, in which n = 5. *, P < 0.01 compares with the GH alone treatment of the same sex; †, P < 0.01 compares women with men exposed to the same hormone treatment. A representative immunoblot of CYP3A4 and its respective loading control (actin) is presented in the figure.