Fig. 2.

Sex-dependent hormonally regulated DNA bound HNF-4 (A), glucocorticoid receptor (GR) (B), and RXR (C) in nuclei from hepatocytes derived from adult men and woman. The cells were exposed to either continuous dexamethasone alone (DEX), continuous GH alone, or both hormones (DEX+GH) for 5 d in culture, after which the hepatocytes were harvested, and transcription factor bound DNA was determined by a TransSignal Protein/DNA Array I kit. The signals were normalized with references provided by the manufacturer. Each data point is a mean ± sd for cells from five individuals, all treatments. *, P < 0.01 compares with the GH alone treatment of the same sex; †, P < 0.01 compares women with men exposed to the same hormone treatment. Representative arrays of HNF-4/DNA, GR/DNA, and RXR/DNA complexes and their respective loading controls (biotinylated DNA) are presented in the figure.