Fig. 1.

Localization of ER by RT-PCR. RNA was extracted from central and peripheral regions of male rat lung and converted to cDNA with reverse transcriptase. Isoform-specific primers were used to amplify the two known ER subtypes by PCR. Integrity of PCR process was verified by the presence of the ribosomal protein L19 mRNA. ER samples were subjected to 40 PCR cycles; L19 levels were determined after 30 cycles. RT-PCR products were electrophoresed on 1.5% agarose gels and stained with ethidium bromide. Images were captured and analyzed with an AlphaImager HP Imaging System.