A, Orexin1R distribution (left panel) and orexin2R distribution (right panel) in membrane and cytosolic fractions of pPVT punches taken from rats that were subjected to no stress (n = 10), 1 d of swim stress (acute swim, n = 10), or 4 d of swim stress (repeated swim, n = 10). The inset is a composite of representative Western blots for all groups. *, P < 0.05 vs. vehicle in cytosolic fraction; #, P < 0.05 vs. vehicle in membrane fraction; **, P < 0.05 acute swim vs. repeated swim in cytosolic fraction. B, Phosphorylated ERK1 (left panel) and ERK2 (right panel) in the cytosolic fraction of pPVT punches taken from rats in A. *, P < 0.05 significantly different pERK2 than in other groups.