Table 1.
Epitope residues for anti-GHRext-mAb and anti-GHRmAb 18.24 in rabbit GHR ECD determined by random PCR and expression screening
| Anti-GHRext-mAb |
Anti-GHRmAb 18.24 |
||
|---|---|---|---|
| Determined epitope residues in GHR sequence | Single-residue mutants with negative immunoresponse | Determined epitope residues in GHR sequence | Single-residue mutants with negative immunoresponse |
| L142 | L142P | L142 | L142S, L142P |
| N143 | N143K | N143 | N143S (2), N143Y |
| S145 | S145G, S145N | S145 | S145R, S145N |
| T147 | T147A | T147 | T147A, T147G |
| H150 | H150Q, H150R | I149 | I149T, I149S |
| I153 | I153V | H150 | H150Q, H150R |
| V155 | V155E | D152 | D152G, D152N |
| D164 | D164N | I153 | I153T |
| V165 | V165I (2) | D164 | D164N, D164Y, D164V |
| K167 | K167R | W169 | W169R (2), W169K |
| G168 | G168S | L172 | L172P, L172S |
| L172 | L172P | E173 | E173K, E173G |
| Y174 | Y174H | E175 | E175G (2) |
| M189 | M189T | T195 | T195L, T195S |
| P191 | P191S | V197 | V197F |
| T195 | T195P | S201 | S201P, S201L, S201E |
| P198 | P198L | R203 | R203G, R203S |
| S201 | S201P, S201L | K206 | K206I, K206R, K206E (2) |
| R203 | R203G, R203S | E207 | E207G |
| V212 | V212E | V212 | V212L, V212F |
| S214 | S214P | R213 | R213K |
| R217 | R217N | S218 | S218G |
| S219 | S219P (2) | S219 | S219F, S219P |
Numbers in parentheses are the frequency for the same residue.