Table 1.
Anti-GHRext-mAb |
Anti-GHRmAb 18.24 |
||
---|---|---|---|
Determined epitope residues in GHR sequence | Single-residue mutants with negative immunoresponse | Determined epitope residues in GHR sequence | Single-residue mutants with negative immunoresponse |
L142 | L142P | L142 | L142S, L142P |
N143 | N143K | N143 | N143S (2), N143Y |
S145 | S145G, S145N | S145 | S145R, S145N |
T147 | T147A | T147 | T147A, T147G |
H150 | H150Q, H150R | I149 | I149T, I149S |
I153 | I153V | H150 | H150Q, H150R |
V155 | V155E | D152 | D152G, D152N |
D164 | D164N | I153 | I153T |
V165 | V165I (2) | D164 | D164N, D164Y, D164V |
K167 | K167R | W169 | W169R (2), W169K |
G168 | G168S | L172 | L172P, L172S |
L172 | L172P | E173 | E173K, E173G |
Y174 | Y174H | E175 | E175G (2) |
M189 | M189T | T195 | T195L, T195S |
P191 | P191S | V197 | V197F |
T195 | T195P | S201 | S201P, S201L, S201E |
P198 | P198L | R203 | R203G, R203S |
S201 | S201P, S201L | K206 | K206I, K206R, K206E (2) |
R203 | R203G, R203S | E207 | E207G |
V212 | V212E | V212 | V212L, V212F |
S214 | S214P | R213 | R213K |
R217 | R217N | S218 | S218G |
S219 | S219P (2) | S219 | S219F, S219P |
Numbers in parentheses are the frequency for the same residue.