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. Author manuscript; available in PMC: 2011 Dec 5.
Published in final edited form as: J Biol Chem. 2006 Dec 19;282(9):6356–6363. doi: 10.1074/jbc.M609658200

Figure 3.

Figure 3

Equilibrium dissociation experiments of tmRNA on programmed ribosomes, measured by filter-binding assays. The maximum of binding that we could obtain, at the highest ribosome concentration, was set to 1, and the other values were measured on the 0 to 1 scale, expressed as “fraction bound”. ◆: 70S-tRNA stalled with a truncated mRNA leaving the A site empty, in the absence of SmpB. ▲: 70S-tRNA stalled with an mRNA bearing a sense codon at the A site, in the presence of SmpB. ■: 70S-tRNA stalled with a truncated mRNA leaving the A site empty, in the presence of SmpB.