Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vivo interaction of SmpB with the small subunit and tmRNA. Crude ribosomes (P100) from E. coli cells were fractionated by sucrose gradient centrifugation at low concentration of Mg2+ ions. (A) Absorbencies of each fraction. (B) 23S and 16S ribosomal RNAs were detected by ethidium bromide staining and were separated on a formaldehyde-agarose gel. (C) Detection of tmRNA by Northern hybridization. (D) Detection of the presence of SmpB protein in sucrose fractions by Western blotting.
