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. Author manuscript; available in PMC: 2011 Dec 5.
Published in final edited form as: Nature. 2010 Nov 24;468(7326):983–987. doi: 10.1038/nature09561

Figure 1.

Figure 1

RecBCD removes RNAP from DNA. a, Distribution of QD-RNAP bound to λ-DNA. Locations of promoters are indicated; those facing left are shown blue, those facing right are red. The inset shows examples of YOYO1-stained λ-DNA (green) bound by RNAP (magenta). The tethered end of the DNA is on the left, and the free end of the DNA is on the right. b, Kymograms of RecBCD colliding with RNAP core, holoenzyme, stalled elongation complex (EC), and stalled ECs chased with rNTPs. Gaps in magenta traces correspond to QD blinking. c, Distribution of event types. d, Tracking data for collisions, with traces aligned at the collisions.