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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Jun 8;50(29):6511–6515. doi: 10.1002/anie.201102909

Figure 1.

Figure 1

Charge segregation in split inteins. a) Sequence logo based on a sequence alignment of 24 naturally split inteins highlighting the highly conserved charged residues. b) Structure of NpuWT determined by NMR spectroscopy (PDB code: 2KEQ) highlighting intermolecular electrostatic interactions. NpuNWT and NpuCWT are shown in black and gray, respectively. Acidic residues are shown in red, basic residues are shown in blue, and terminal catalytic residues are shown in orange. c) Comparison of the calculated isoelectric points (pI) for the N- and C-terminal fragments of 24 naturally split inteins, the N- and C-terminal sequences of 76 intact inteins, and the mutant split intein (NpuMUT) engineered in this study. NpuWT and NpuMUT are both indicated on the plot as red circles.