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Rational design strategy for charge-swapped inteins. a) Scheme depicting the desired specificity in charge-swapped inteins. b) Dose–response curves for NpuWT and NpuMUT fragment combinations in an in vivo intein activity-coupled kanamycin resistance assay. Mutation clusters in NpuMUT are (E7K, K130E), (E52K, E54K, K113E), (D85K, E89R, R108E), and (E61K, E91K, K104E).
