Step #	Volume (mL)	Description	Parameters
1	0.00	Collect 1.0 mL fractions during entire run
2	0.00	Switch Columns	HIC Column 1 (Position 2)
3	0.00	Isocratic Flow	pH: 6.80 100% B	Volume: 2.00 mL Flow: 1.00 mL/min
4	2.00	Zero Baseline	QuadTec
5	2.00	Zero Baseline	UV Detector
6	2.00	Inject Sample	Sample Load Dynamic Loop	Auto Inject Valve Volume: 0.50 mL Flow: 1.00 mL/min
7	3.00	Isocratic Flow	pH: 6.80 100%B	Volume: 5.00 mL Flow: 1.00 mL/min
8	8.00	Linear Gradient	pH: 6.80 100%B -> 0%B	Volume: 10.00 mL Flow: 1.00 mL/min
9	18.00	Isocratic Flow	pH: 6.80 0%B	Volume: 3.00 mL Flow: 1.00 mL/min
10	21.00	Isocratic Flow	pH: 6.80 100%B	Volume: 5.00 mL Flow: 1.00 mL/min
11	26.00	Switch Columns	Union (Position 1)
End	26.00	End of Protocol	Automatically Repeats with 5 additional HIC columns (Positions 3-7)
Scout type	Number of Runs: 6	Number of Steps Scouted: 1