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. 2011 Sep 15;(55):3200. doi: 10.3791/3200
After dissociation without Lectin-based preplating
Total number of cells/SC Trypan blue-positive cells [%] Islet-1/2- positive cells [%]
1 995.0 13,1 9,5
2 889.4 8,0 10,0
3 1.180.0 11,0 10,8
Mean ± SD 1.021.0 ± 147.1 10,7 ± 2,6 10,1 ± 0,7
With lectin-based preplating
Number of cells after preplating/SC Trypan blue-positive cells [%] Islet-1/2- positive cells [%]
1 189.6 9,9 70,5
2 168.8 3,7 76,1
3 125.0 0,0 72,5
Mean ± SD 161.1 ± 33.0 4,5 ± 5,0 73,0 ± 2,8