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Structural characterization of nonasaccharide 8. (A) DEAE-HPLC chromatogram of 35S-labeled nonasaccharide. (B) The structure of nonasaccharide 8. (C) The RPIP-HPLC chromatogram of the disaccharides of heparin lyase-digested N-[35S]sulfo nonasaccharide 8. (D) The reaction involved in the digestion of nonasaccharide 8 by heparin lyases. A nonradioactive monosaccharide was formed during the digestion; however, it was not detectable.
