Figure 4.
The effect of Dex, IL-10, and TA on caspase-5 protein production and maturation by IL-1β (A) and LPS (B, C) in hRPE cells. hRPE cells were pretreated with Dex (10−8 or 10−7 to 10−6 M), IL-10 (100 U/mL), or TA (0.1 or 0.01 mg/mL) for 30 minutes and then coincubated with IL-1β (2 ng/mL) or LPS (1000 ng/mL) for an additional 24 hours. Proteins from the whole hRPE cell lysates were detected by anti–caspase-5 antibody specific for pro-caspase-5 and cleaved caspase-5. Fold changes of the cleaved caspase-5 were calculated by relative density between treated and untreated samples, as determined by densitometry after normalization with actin protein.