Table 4.

Validation of the gene expression profiling data by quantitative real-time PCR

8b2			8b2 + docetaxel		1-INT		1-INT + docetaxel
Gene	Affy	QPCR	Affy	QPCR	Affy	QPCR	Affy	QPCR
SERPINE1	−11.2	−4.55 ± 0.3, p < 0.0001	−15.62	−18.89 ± 2.1, p < 0.0001	−8.04	−6.86 ± 1.25, p = 0.0005	−12.75	−12.53 ± 1.45, p = 0.0001
CXCL5	−4.04	−3.0 ± 0.24, p = 0.0132	−5.11	−3.8 ± 0.15, p < 0.0001	−3.11	−2.47 ± 0.13, p = 0.0023	−4.3	−2.71 ± 0.44, p < 0.0001
TGFBI	−7.38	−6.9 ± 2.32, p = 0.0008	−8.38	−5.83 ± 0.02, p < 0.0001	−2.25	−2.84 ± 0.3, p = 0.0001	−2.45 −3.01	± 0.5, p < 0.0001
GBP1	−9.93	−5.27 ± 0.3, p = 0.0046	−9.73	−5.76 ± 0.73, p < 0.0001	−7.03	−7.22 ± 0.4, p = 0.0007	−8.08	−4.98 ± 0.52, p = 0.0018
CYP1B1	12.43	6.62 ± 0.11, p = 0.0009	13.83	13.49 ± 2.42, p = 0.0004	5.99	3.4 ± 0.75, p = 0.0008	9.19	5.29 ± 0.56, p = 0.0002
KCNMA1	7.09	4.7 ± 0.52, p < 0.0001	6.22	5.39 ± 0.8, p = 0.0004	7.7	4.62 ± 1.62, p = 0.0001	6.77	2.43 ± 0.2, p = 0.0004
CCNG2	2.52	3.64 ± 0.48, p = 0.0017	2.98	4.33 ± 0.31, p < 0.0001	3.51	5.3 ± 2.03, p = 0.0006	4.01	8.95 ± 1.6, p = 0.0028
HES1	2.80	5.81 ± 1.48, p = 0.0053	2.38	4.18 ± 0.19, p = 0.0006	2.67	4.88 ± 0.59, p = 0.0001	2.43	4.73 ± 0.62, p = 0.0078

Randomly selected genes showing differential pattern of expression by gene arrays (affy) were measured by quantitative real-time PCR (qRT-PCR) and showed fold change values consistent with gene arrays. QPCR values are mean ± s.e.m and the p values were calculated using two-tailed student's t-test.