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. 2011 Sep 6;30(21):4489–4499. doi: 10.1038/emboj.2011.319

Figure 2.

Figure 2

Dlx2 protects from TGFβ-induced cell-cycle arrest and apoptosis. (A) Confocal laser scanning microscopy of NMuMG cells stably expressing N-terminal HA-tagged Dlx2. Dlx2 was detected by anti-HA immunofluorescence staining (green). Blue DAPI staining visualizes nuclei. Scale bar=100 μm. (B) Dlx2-expressing (Dlx2) and control (CTR) NMuMG cells were treated with or without TGFβ for the days indicated and counted by trypan blue exclusion using a Neubauer cell counting chamber. The time point day 6 was used to determine statistical significance between Dlx2-expressing and control cells. (C) Dlx2-expressing and control NMuMG cells were treated with TGFβ for the days indicated. Apoptosis was measured by Annexin V staining and flow cytometry. (D) Dlx2-expressing and control NMuMG cells were treated with TGFβ for the days indicated, and proliferation rates were determined by BrdU incorporation and flow cytometry. Data are shown as mean values±s.d. and are representative of three independent experiments. Statistical values are calculated by using an unpaired, two-tailed t-test. *P⩽0.05; **P⩽0.01.