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. 2011 Sep 30;30(21):4414–4422. doi: 10.1038/emboj.2011.359

Figure 1.

Figure 1

miR-671 represses CDR1 expression. (A) Schematic representation of the CDR1 locus. CDR1 mRNA (chrX: 139,693,091–139,694,389, hg18) and the antisense RNA (chrX: 139,693,005–139,694,491, hg18) derived from EST accessions (for additional detail, see Supplementary Figure S3). The enlargement shows the miR-671 antisense target region along with vertebrate conservation from UCSC Genome Browser (17-way conservation). (B) CDR1 expression in HEK293-eGFP-671 cells at indicated time points from 0 to 40 days of tetracycline induction. Expression was determined by qRT–PCR on random hexamer primed total RNA using the primer set CDR1(4)FW/CDR1(4)RE (normalized to 18S, relative to uninduced levels). (C) Northern blot showing subcellular localization of miR-671 in nuclear and cytoplasmic fractions of HEK293 cells transiently transfected with pJEBB-671. miR-15b is included as a localization control for an endogenously expressed miRNA and U6 snRNA and tRNALys are nuclear- and cytoplasm-specific RNAs, respectively. (D) CDR1 expression in HEK293 cells transfected with anti-mir-671 or scrambled control determined by qPCR (as in B).