Figure 3.

Localization of Mcs proteins by immuno-EM. Cells expressing C-terminally HA-tagged versions of Mcs proteins, were processed for immuno-EM, cryosections were labelled with anti-HA antibodies and protein A bound gold particles, with the exception of Por1, for which specific antibodies were used. (A) Distribution of Mcs proteins in cells grown on lactate. (B) Mcs10 localization in cells grown on glycerol. (C) Distribution of proteins of various mitochondrial subcompartments in cells grown on lactate. Matrix (Idh1); OM, (Por1, Tob38 and Mas37/Tob37); inner boundary and crista membrane (Tim50 and Tim16). CW, cell wall; M, mitochondrion; N, nucleus; PM, plasma membrane; V, vacuole. Size bars, 200 nm. Additional examples in Supplementary Figure S3. (D) Quantitative analysis of the distribution of Mcs and control proteins at sites where the CJ meet the IBM. For each labelling, the percentage of gold particles present at the CJ was determined.