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. 2011 Aug 1;12(3):215–228. doi: 10.4161/cbt.12.3.16218

Figure 2.

Figure 2

SRC family kinases play a key role in the regulation of the ERK1/2 pathway by CHK1 inhibitors. (A) Transformed mouse embryonic fibroblasts, MEF (wild type, WT; deleted for SRC/FYN/YES, SYF) were treated with vehicle (VEH, DMSO), UCN-01 (100 nM) or AZD7762 (50 nM). Cells were isolated at the indicated time points and subjected to SDS PAGE followed by immunoblotting to determine the phosphorylation of ERK1/2 (P-ERK1/2), the phosphorylation of SRC Y461 (P-Y416 Src) or the expression of GAPDH. Data are from a representative of three separate studies. (B) MCF7 cells were treated with vehicle (VEH, DMSO), UCN-01 (100 nM), PP2 (10 µM), AZD7762 (50 nM) or UCN-01+PP2 or AZD7762+PP2. Cells were isolated 2 h after drug exposure and subjected to SDS PAGE followed by immunoblotting to determine the phosphorylation of ERK1/2 (P-ERK1/2) or the expression of ERK2 protein. Data are from a representative of two separate studies.