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. 2011 Feb 1;10(3):481–491. doi: 10.4161/cc.10.3.14713

Figure 9.

Figure 9

Dominant-negative AKT reduces ERK phosphorylation in response to IR and EcoRI-induced DSBs. HEK293 cells stably expressing pcDNA3 or DN-AKT-Myc plasmids were: (A) exposed to 0, 5 or 10 Gy and collected after 10 min for western blotting and subsequent densitometric analysis. ERK denotes equal loading. Myc indicates expression of DN-AKT. Data points, phosphorylation levels plotted against radiation dose normalized to ERK or (B) electroporated with either buffer alone or buffer with EcoRI enzyme (2.5 units) and cells collected after 5 min for western blotting and subsequent densitometric analysis. Data points, ERK phosphorylation levels plotted against buffer alone or EcoRI normalized to ERK. Error bars, SEM; n = 3. Fold (x) denotes changes in p-ERK levels compared to control (pcDNA3, no EcoRI) normalized to ERK. *p < 0.05.