Table 3.
PCR primers and conditions for sequencing of DPF2
| Exon | Primer sequence (5′- 3′) | Length (bp) | Temp (°C) | PCR Temp.°C | Product length (bp) |
|---|---|---|---|---|---|
| 1 | AGTCGCAACAAGGCTTTCTCGGG | 23 | 62 | 62 | 306 |
| GCCCCTGTGGACAGTCACGAAGC | 23 | 64 | |||
| 2–4 | GAAGCCAGTCAGTATCCAGTGC | 22 | 58 | 63 | 1,516 |
| TCCCGGTCCCTTGAGCCTTCC | 21 | 64 | |||
| 5–6 | ATCGCTCCTATCCCCATGCCATTC | 24 | 61 | 63 | 1,269 |
| GTTTGATTCGGCCCCTTGGAGATG | 24 | 60 | |||
| 7–9 | CAAGCAAGTCCTCCCTGGGGTGTG | 24 | 64 | 63 | 720 |
| TGGCGGGGACATGGCACTCTT | 21 | 64 | |||
| 10 | AGTTAAACCGCTCCTTCTGG | 20 | 55 | 62 | 516 |
| ACGGCAGCCTGAATTTCCTATCCA | 24 | 61 | |||
| 11 and 3′UTR | CTTTACATATTGGGCTTTAGCA | 22 | 51 | 61 | 1,691 |
| TCAAGAGGGCCTAGGAGTGTATTT | 24 | 57 |