Figure 3.

Examples of antibody specificity analysis. The upper part of each panel shows the image of the array. Peptide spots are annotated on the left copy of the duplicates, color coded as described below the image. On the left side, spots are annotated as follows: all peptides containing the primary PTM are shaded in green, false negatives (or very weakly bound peptides which contain the primary PTM) are highlighted with red arrows. False positives are encircled in orange or violet color. The secondary PTMs present in the false negative, and false positive spots are specified below the pictures. In the lower part a scatter plot of the binding intensities to both repeats is shown.