Figure 5.

(A) Histograms of the unbinding forces for the p28/DBD complex before and after blocking. The most probable unbinding force value was determined from the maximum of the main peak of the histogram before blocking. All measurements were performed at a loading rate of 7 nN/s. (B) Histogram of the unbinding lengths for the p28/DBD interaction, evaluated for same collection of force curves as in (A). The continuous line is the fit by a Gaussian centered at 12.1 nm and with a standard deviation of 3.7 nm.

Abbreviation: DBD, DNA-binding domain.