Figure 5. Quantitative PCR of RNA from lung cells demonstrates a decrease in negative regulators of Th1 immunity in Mtb challenged vaccinated rats.

A) Total RNA from lung cells of Mtb challenged BCG vaccinated and unvaccinated rats (N = 5) was isolated at 15 days post-challenge and the relative expression levels for all 84 genes in the two groups are plotted against each other in the scatter plot. Genes encoding Socs1, Irf4, NF-κβ1 and JunB are down-regulated by at least two-fold in BCG vaccinated rats relative to unvaccinated rats (green circles). B) Cluster-diagram showing differential expression pattern of four of the above mentioned genes in individual rat belonging to the two treatment groups.