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. 2011 Dec 5;6(12):e28368. doi: 10.1371/journal.pone.0028368

Figure 2. Molecular cytogenetics of relevant chromosomes in wild-type, fully ciprofloxacin-resistant, and revertant macrophages.

Figure 2

mFISH and FISH experiments (red: control probe; green: Abcc4 probe) for relevant chromosomes in wild-type macrophages, in the clones of the fully ciprofloxacin-resistant macrophages CR200, and in the revertant macrophages (Rev200). Karyotypes of the cell lines (abnormalities illustrated in the figure are highlighted in bold): a) wild-type cells: 72<3n>,X,der(X)T(X;11)(E or F1;?B5),der(1)T(1;6)(C?;B?3), +der(1)(1A1→1C?::6B?3→6D∼F::X? →X?),−3,+der(5;17)(5A1→5C2::17A1→17?), der(6)T(1;6)(?D;B3),+der(6),+der(9)T(9;19)(?B;C2),+?Del(12)(?B),−13,idic(13), der(14)T(3;14)(E2;C?1),idic(15),−18,−19,idic(19),ace(3)x2,ace(18)x2; b) CR200 cells (“idem” refers to the chromosomal abnormalities stated in the wild-type cells karyotype): – clone I: 72,idem,Is(5;14)(B;?),+9,−der(9)T(9;19),+13,−idic(13),ace(3)x2∼3, ace(?14)x0∼2,ace(18)x1∼2[cp17]; – clone II: 72,idem,?+9,−der(9)T(9;19),+13,−idic(13),mar1x2∼3, ace(?14)x1∼2,ace(18)x1∼2[cp3]; – clone III: 71,idem,+der(1)T(1;2)(H?;?),−der(9)T(9;19),+13, −idic(13),der(16)T(14;16)(?;C4),ace(3)x3,ace(?14)x0∼2[cp2].