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. 2011 Dec 5;6(12):e28509. doi: 10.1371/journal.pone.0028509

Figure 1. Silencing of STAT6 in NCI-H460 cells using STAT6 specific siRNA.

Figure 1

a) The western blot of STAT6 and phosphorylated form of STAT6 (pSTAT6) protein on cell extracts from untransfected, STAT6 specific siRNA and scrambled siRNA transfected NCI-H460 cells at different time points as indicated. b) Graph represents the fold change in STAT6 mRNA level, STAT6 protein and pSTAT6 protein level compared to untransfected NCI-H460 cells at respective time points. Scrambled siRNA at different time points was used as a negative control. GAPDH was used as a loading control for densitometric analysis for western blot analysis. The mRNA levels were normalized to 18s rRNA expression in Real time PCR analysis. The data is expressed as the mean ± S.D. of 3 independent experiments. * indicates p value < 0.05 in comparison to untransfected cells. ** indicates p value < 0.01 in comparison to untransfected cells. c).